Find animal-free science methods

Despite their availability, the majority of reliable in chemico, in vitro or in silico non-animal methods are not widely used in science and are not yet accepted to fulfill regulatory requirements. There are several reasons for this, including a lack of familiarity with existing non-animal methods or simply a lack of awareness. To overcome this challenge, Animal Free Science endorses knowledge to help scientists become more proficient in the field in chemico, in vitro or in silico methods.

The overview below lists some useful websites covering non-animal methods in science and education. This list includes freely available online courses and webinars.

Please note that Animal Free Science cannot be held responsible for any material or the security of any of the websites listed.


    Bibliography on Alternatives to the Use of Live Vertebrates in Biomedical Research and Testing from the United States National Library on Medicine. Note: searches PubMed/Medline.


    Altweb, the Alternatives to Animal Testing Web Site, was created to serve as a gateway to alternatives news, information, and resources on the Internet and beyond. Altweb now is the U.S. home of the journal ALTEX: Alternatives to Animal Experimentation, which is the official publication of the Johns Hopkins Center for Alternatives to Animal Testing (CAAT).

  • EnviroTox database

    The EnviroTox database is an international project, led by the Health and Environmental Sciences Institute and involving the JRC. This public database of ecotoxicological data brings together more than 91,000 curated records for more than 4000 chemicals across 1500 species.


    Official website of the European Reference Laboratory for Alternatives to Animal Testing .


    The DB-ALM is a service that provides information on development and applications of advanced and alternative methods to animal experimentation in the field of biomedical sciences and toxicology for research or regulatory purposes.

  • Fetal Calf Serum (FCS)-free Database

    This useful research tool provides an overview of non-fetal-calf-serum media for use in cell culture.

  • NORINA (Norwegian Inventory of Audiovisuals)

    Database containing information on over 3,800 audiovisual aids that may be used as alternatives or supplements to the use of animals in teaching and training, including dissection alternatives, at all levels from junior school to University.

  • RE-Place

    The RE-Place project aims to centralize the expertise present in the Flemish and Brussels regions in a database and make it more accessible to the general public.

  • Tissue Solutions

    Tissue Solutions is a customer-focused provider of ethically sourced human samples required for preclinical drug development and research.


    A collection of databases produced by the National Library of Medicine covering toxicology, hazardous chemicals, environmental health and related areas.

  • Altex

    The journal ALTEX – Alternatives to Animal Experimentation publishes open access academic articles on the development and implementation of alternatives to the use of animals for scientific purposes and informs on international developments in this field.

  • ATLA

    ATLA is published by FRAME, and has been a key journal in the field of laboratory animal alternatives for more than 30 years

  • Organs-On-A-Chip

    Organs-on-a-Chip is a new interdisciplinary journal dedicated to publishing research and development in the field of organs, tissues and organoids on chips and their application.

  • Toxicology in Vitro

    Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling.

  • PubMed

    Developed by the National Center for Biotechnology Information (NCBI) at the National Library of Medicine (NLM). Provides access to citations from biomedical literature, access to full-text articles at journal Web sites and other related Web resources, and access and links to the other molecular biology resources.

  • Altertox Academy
  • Altertox Academy is about understanding new toxicological models, practicing side-by-side with experts and integrating new tools to your daily working environment or your future position. They organise regular toxicology trainings in Belgium and throughout Europe to increase expertise on the regulatory context of REACH, Cosmetics ban, EU Pharmacopoeia and much more.

  • FRAME Training school
  • FRAME training schools provide training in experimental design and statistics and are often held in association with universities throughout the UK, Europe and Scandinavia.

Training scheduled on a specific date and location will be listed in the Agenda section. Contact us if you want us to share your online course or webinar.